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Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between <t>RFC10</t> prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.
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Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between <t>RFC10</t> prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.
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Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between <t>RFC10</t> prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.
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Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between <t>RFC10</t> prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.
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Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between <t>RFC10</t> prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.
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Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between <t>RFC10</t> prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.
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Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between <t>RFC10</t> prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.
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Top row: log-scale reflectance and DOPU of albino mouse retina. Second row: log-scale reflectance and DOPU of C57BL/6 mouse retina. Third row: log-scale reflectance and DOPU of <t>Cx3cr1</t> GFP <t>Ccr2</t> RFP mouse retina. Retinal layers as indicated on reflectance image in top row: retinal nerve fibre layer (RNFL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); external limiting membrane (ELM); photoreceptor outer segments (OS); photoreceptor inner segments (IS); retinal pigment epithelium (RPE); choroid.
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cx3cr1 gfp ccr2 rfp mice  (Charles River Laboratories)
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Top row: log-scale reflectance and DOPU of albino mouse retina. Second row: log-scale reflectance and DOPU of C57BL/6 mouse retina. Third row: log-scale reflectance and DOPU of <t>Cx3cr1</t> GFP <t>Ccr2</t> RFP mouse retina. Retinal layers as indicated on reflectance image in top row: retinal nerve fibre layer (RNFL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); external limiting membrane (ELM); photoreceptor outer segments (OS); photoreceptor inner segments (IS); retinal pigment epithelium (RPE); choroid.
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Image Search Results


Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between RFC10 prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.

Journal: STAR Protocols

Article Title: Protocol for condensate-based stabilization of gene circuit dynamics under growth-mediated dilution in E. coli

doi: 10.1016/j.xpro.2026.104536

Figure Lengend Snippet: Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between RFC10 prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.

Article Snippet: BglII-RFP- RFC10 surfix , E1010 , RFP-BglII-F , VR , 880 bp.

Techniques: Sequencing, Construct, Activation Assay, Control

Top row: log-scale reflectance and DOPU of albino mouse retina. Second row: log-scale reflectance and DOPU of C57BL/6 mouse retina. Third row: log-scale reflectance and DOPU of Cx3cr1 GFP Ccr2 RFP mouse retina. Retinal layers as indicated on reflectance image in top row: retinal nerve fibre layer (RNFL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); external limiting membrane (ELM); photoreceptor outer segments (OS); photoreceptor inner segments (IS); retinal pigment epithelium (RPE); choroid.

Journal: Biomedical Optics Express

Article Title: Histology correlated adaptive optics polarisation sensitive optical coherence tomography

doi: 10.1364/BOE.590238

Figure Lengend Snippet: Top row: log-scale reflectance and DOPU of albino mouse retina. Second row: log-scale reflectance and DOPU of C57BL/6 mouse retina. Third row: log-scale reflectance and DOPU of Cx3cr1 GFP Ccr2 RFP mouse retina. Retinal layers as indicated on reflectance image in top row: retinal nerve fibre layer (RNFL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); external limiting membrane (ELM); photoreceptor outer segments (OS); photoreceptor inner segments (IS); retinal pigment epithelium (RPE); choroid.

Article Snippet: Cx3cr1 GFP Ccr2 RFP transgenic mice (Jackson Laboratory, strain no. 032127; RRID: IMSR_JAX:032127) were used to generate time-course images.

Techniques: Membrane

Top row: log-scale reflectance and DOPU of albino mouse retina. Second row: log-scale reflectance and DOPU of C57BL/6 mouse retina. Third row: log-scale reflectance and DOPU of Cx3cr1 GFP Ccr2 RFP mouse retina. Retinal layers as indicated on reflectance image in top row: retinal nerve fibre layer (RNFL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); external limiting membrane (ELM); photoreceptor outer segments (OS); photoreceptor inner segments (IS); retinal pigment epithelium (RPE); choroid.

Journal: Biomedical Optics Express

Article Title: Histology correlated adaptive optics polarisation sensitive optical coherence tomography

doi: 10.1364/BOE.590238

Figure Lengend Snippet: Top row: log-scale reflectance and DOPU of albino mouse retina. Second row: log-scale reflectance and DOPU of C57BL/6 mouse retina. Third row: log-scale reflectance and DOPU of Cx3cr1 GFP Ccr2 RFP mouse retina. Retinal layers as indicated on reflectance image in top row: retinal nerve fibre layer (RNFL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); external limiting membrane (ELM); photoreceptor outer segments (OS); photoreceptor inner segments (IS); retinal pigment epithelium (RPE); choroid.

Article Snippet: Cx3cr1 GFP Ccr2 RFP mice are derived from a C57BL/6 genetic background.C57BL/6J mice (Charles River, strain no. 027; RRID: IMSR_CRL:027) and albino (Charles River, strain number 022, Crl:CD1(ICR)) mice were used for validation.

Techniques: Membrane